Secondary Antibodies
Secondary antibodies bind to a primary antibody to assist in detection, amplification, sorting, and purification of target antigens, and are used in various biochemical techniques/assays (e.g., ELISA, western blot (WB), immunohistochemistry).
Goat Secondary Antibodies
(44,210)
Mouse Secondary Antibodies
(14,620)
Rabbit Secondary Antibodies
(6,299)
Donkey Secondary Antibodies
(4,319)
Rat Secondary Antibodies
(1,234)
Sheep Secondary Antibodies
(701)
Chicken Secondary Antibodies
(403)
Bovine Secondary Antibodies
(257)
Pig Secondary Antibodies
(77)
Horse Secondary Antibodies
(74)
Alpaca Secondary Antibodies
(62)
Hamster Secondary Antibodies
(57)
Biosimilar Secondary Antibodies
(9)
Human Secondary Antibodies
(7)
Duck Secondary Antibodies
(3)
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| Content And Storage | 4°C, do not freeze |
|---|---|
| Target Species | Rat |
| Host Species | Mouse |
| Conjugate | PE |
| Applications | Flow Cytometry |
| Form | Liquid |
| Isotype | IgG1 |
| Concentration | 0.1 mg/mL |
| Antigen | Rat IgG2b |
| Regulatory Status | RUO |
| Purification Method | Protein G |
| Product Type | Secondary Antibody |
| Formulation | PBS with 4-5mg/mL BSA and 0.02% sodium azide |
| Immunogen | Rat IgG. |
| Classification | Monoclonal |
| Primary or Secondary | Secondary |
| Clone | NORIG 7.16.2 |
Guinea Pig anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed), Novus Biologicals™
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Guinea Pig Polyclonal Antibody has been used in 3 publications
| Target Species | Rabbit |
|---|---|
| Host Species | Guinea Pig |
| Conjugate | Unconjugated |
| Applications | ELISA,Immunohistochemistry,Western Blot |
| Isotype | IgG |
| Research Discipline | Adaptive Immunity, Immunology |
| Concentration | 1.2 mg/ml |
| Antigen | Rabbit IgG, H/L Chains |
| Regulatory Status | RUO |
| Purification Method | Affinity Purified |
| Dilution | Western Blot 1:2000 - 1:10000, ELISA 1:20000 - 1:100000, Immunohistochemistry 1:1000 - 1:5000 |
| Classification | Polyclonal |
| Immunogen | Rabbit IgG whole molecule |
| Primary or Secondary | Secondary |
| Test Specificity | This antibody was pre-adsorbed against Human, Goat, and Mouse Serum Proteins. No reaction was observed against Goat, Human and Mouse Serum Proteins. |
Mouse anti-Rabbit IgG (H+L) Secondary Antibody, Allophycocyanin, Novus Biologicals™
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Mouse Polyclonal Antibody
| Content And Storage | Store at 4°C in the dark. |
|---|---|
| Target Species | Rabbit |
| Host Species | Mouse |
| Conjugate | APC |
| Applications | Dot Blot,ELISA,Immunohistochemistry,Western Blot |
| Isotype | IgG |
| Research Discipline | Adaptive Immunity, Immunology |
| Antigen | IgG (H+L) |
| Regulatory Status | RUO |
| Purification Method | Multi-step |
| Formulation | PBS |
| Classification | Polyclonal |
| Immunogen | Mouse anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) was produced by repeated immunization with rabbit whole IgG molecule in mouse. |
| Primary or Secondary | Secondary |
Mouse anti-Human IgG Fc Secondary Antibody (3D3cc) [Janelia Fluor 669], Novus Biologicals™
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Mouse Monoclonal Antibody
| Content And Storage | Store at 4°C in the dark. |
|---|---|
| Target Species | Human |
| Host Species | Mouse |
| Conjugate | Janelia Fluor 669 |
| Applications | Western Blot,Immunodiffusion,Sandwich ELISA |
| Form | Purified |
| Isotype | IgG2a |
| Antigen | IgG Fc |
| Regulatory Status | RUO |
| Purification Method | Protein A purified |
| Formulation | 50mM Sodium Borate |
| Classification | Monoclonal |
| Immunogen | Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with IgG from human serum. |
| Primary or Secondary | Secondary |
| Clone | 3D3cc |
Mouse anti-Human IgG1 Fc Secondary Antibody (2C11cc) [Janelia Fluor 525], Novus Biologicals™
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Mouse Monoclonal Antibody
| Content And Storage | Store at 4°C in the dark. |
|---|---|
| Target Species | Human |
| Host Species | Mouse |
| Conjugate | Janelia Fluor 525 |
| Applications | ELISA,Immunohistochemistry,Immunohistochemistry (Frozen),Immunodiffusion |
| Form | Purified |
| Isotype | IgG1 |
| Antigen | IgG1 Fc |
| Regulatory Status | RUO |
| Purification Method | Protein A purified |
| Formulation | 50mM Sodium Borate |
| Classification | Monoclonal |
| Immunogen | Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with IgG from human serum. |
| Primary or Secondary | Secondary |
| Clone | 2C11cc |
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BNP Antibody (50E1cc), Janelia Fluor™ 549, Novus Biologicals™
BNP Antibody (50E1cc), Janelia Fluor™ 549, Novus Biologicals™
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Mouse Monoclonal Antibody
| Content And Storage | Store at 4°C in the dark. |
|---|---|
| Target Species | Human |
| Host Species | Mouse |
| Conjugate | Janelia Fluor 549 |
| Applications | ELISA,Immunoassay,Sandwich ELISA,Western Blot |
| Form | Purified |
| Isotype | IgG1 |
| Research Discipline | Biologically Active Proteins, Cardiovascular Biology, Cytokine Research, Immunology, Neuroscience, Neurotransmission |
| Antigen | BNP |
| Regulatory Status | RUO |
| Purification Method | Protein A purified |
| Dilution | ELISA, Sandwich ELISA |
| Gene Alias | BNP, brain type natriuretic peptide, Gamma-brain natriuretic peptide, natriuretic peptide B, natriuretic peptide precursor B, natriuretic peptides B, natriuretic protein |
| Gene ID (Entrez) | 4879 |
| Formulation | 50mM Sodium Borate |
| Classification | Monoclonal |
| Immunogen | Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with synthetic human BNP, whole molecule, conjugated with carrier protein. |
| Primary or Secondary | Primary |
| Clone | 50E1cc |
Mouse anti-Human IgG (H+L) Secondary Antibody (4D2D9G8), Novus Biologicals™
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Mouse Monoclonal Antibody
| Content And Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
|---|---|
| Target Species | Human |
| Host Species | Mouse |
| Conjugate | Unconjugated |
| Applications | ELISA,Western Blot |
| Form | Purified |
| Isotype | IgG1 |
| Concentration | 1.0 mg/mL |
| Antigen | IgG (H+L) |
| Gene Symbols | IGHG1 |
| Regulatory Status | RUO |
| Purification Method | Protein G purified |
| Dilution | Western Blot 1:500 - 1:2000, ELISA 1:10000 |
| Gene Alias | immunoglobulin heavy constant gamma 1 (G1m marker) |
| Gene ID (Entrez) | 3500 |
| Classification | Monoclonal |
| Immunogen | Human IgG was isolated from human sera and purified by chromatography. |
| Primary or Secondary | Secondary |
| Test Specificity | Monoclonal anti-Human IgG is derived from a hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. It is specific for the whole human IgG molecule, and has no cross reactivity with human IgM molecule as determined by an ELISA. |
| Clone | 4D2D9G8 |
Mouse anti-Bovine IgG2 Heavy Chain Secondary Antibody (IL-A73), DyLight 594, Novus Biologicals™
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Mouse Monoclonal Antibody
| Content And Storage | Store at 4°C in the dark. |
|---|---|
| Conjugate | DyLight 594 |
| Target Species | Bovine |
| Host Species | Mouse |
| Applications | ELISA,Flow Cytometry,Immunoprecipitation |
| Form | Purified |
| Isotype | IgG2a |
| Antigen | IgG2 Heavy Chain |
| Regulatory Status | RUO |
| Purification Method | Protein A purified |
| Formulation | 50mM Sodium Borate |
| Classification | Monoclonal |
| Immunogen | Purified bovine IgG2 |
| Primary or Secondary | Secondary |
| Clone | IL-A73 |
Mouse anti-Canine IgA/IgG/IgM (H+L) Secondary Antibody (K99 2E3) [Janelia Fluor 669], Novus Biologicals™
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Mouse Monoclonal Antibody
| Content And Storage | Store at 4°C in the dark. |
|---|---|
| Target Species | Canine |
| Host Species | Mouse |
| Conjugate | Janelia Fluor 669 |
| Applications | ELISA |
| Form | Purified |
| Isotype | IgG1 |
| Antigen | IgA/IgG/IgM (H+L) |
| Regulatory Status | RUO |
| Purification Method | Protein A purified |
| Formulation | 50mM Sodium Borate |
| Classification | Monoclonal |
| Immunogen | Canine immunoglobulin |
| Primary or Secondary | Secondary |
| Clone | K99 2E3 |
Mouse anti-Rat IgG2b Heavy Chain Secondary Antibody (MARG2b-8), PerCP, Novus Biologicals™
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Mouse Monoclonal Antibody
| Content And Storage | Store at 4°C in the dark. |
|---|---|
| Conjugate | PerCP |
| Target Species | Rat |
| Host Species | Mouse |
| Applications | ELISA |
| Form | Purified |
| Isotype | IgG1 |
| Antigen | IgG2b Heavy Chain |
| Regulatory Status | RUO |
| Purification Method | Affinity purified |
| Formulation | PBS |
| Classification | Monoclonal |
| Immunogen | IR863 rat IgG2b myeloma protein |
| Primary or Secondary | Secondary |
| Clone | MARG2b-8 |
IgM (H+L) Mouse anti-Rabbit, DyLight 488, Novus Biologicals™
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Mouse Monoclonal Antibody
| Content And Storage | Store at 4°C in the dark. |
|---|---|
| Conjugate | DyLight 488 |
| Target Species | Rabbit |
| Host Species | Mouse |
| Applications | Flow Cytometry,Immunohistochemistry,Immunohistochemistry (Frozen) |
| Form | Purified |
| Isotype | IgG1 |
| Antigen | IgM (H+L) |
| Regulatory Status | RUO |
| Purification Method | Protein A purified |
| Formulation | 50mM Sodium Borate |
| Classification | Monoclonal |
| Immunogen | Rabbit IgM |
| Primary or Secondary | Secondary |
| Clone | NRBM |
| Content And Storage | Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody. |
|---|---|
| Target Species | Mouse |
| Host Species | Mouse |
| Conjugate | RB613 |
| Applications | Flow Cytometry |
| Form | Liquid |
| Isotype | IgG2b κ |
| Concentration | 0.2 mg/mL |
| Regulatory Status | RUO |
| Gene Alias | Igh-5a; Igh-5.4; Ighd; Immunoglobulin D |
| Product Type | Antibody |
| Gene ID (Entrez) | 380797 |
| Formulation | Aqueous buffered solution containing ≤0.09% sodium azide. |
| Classification | Monoclonal |
| Immunogen | BALB/c mouse splenocytes |
| Primary or Secondary | Secondary |
| Clone | AMS 9.1 |
Mouse anti-Canine IgG2 Heavy Chain Secondary Antibody (CA4F1) [Janelia Fluor 669], Novus Biologicals™
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Mouse Monoclonal Antibody
| Content And Storage | Store at 4°C in the dark. |
|---|---|
| Target Species | Canine |
| Host Species | Mouse |
| Conjugate | Janelia Fluor 669 |
| Applications | Western Blot,Flow Cytometry,ELISA |
| Form | Purified |
| Isotype | IgG1 |
| Antigen | IgG2 Heavy Chain |
| Regulatory Status | RUO |
| Purification Method | Protein A purified |
| Formulation | 50mM Sodium Borate |
| Classification | Monoclonal |
| Immunogen | Canine IgG |
| Primary or Secondary | Secondary |
| Clone | CA4F1 |
Mouse anti-Human IgG1 Fc Secondary Antibody (2C11cc), FITC, Novus Biologicals™
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Mouse Monoclonal Antibody
| Antigen | IgG1 Fc |
|---|---|
| Regulatory Status | RUO |
| Content And Storage | Store at 4°C in the dark. |
| Target Species | Human |
| Host Species | Mouse |
| Conjugate | FITC |
| Applications | ELISA,Immunodiffusion,Immunohistochemistry,Immunohistochemistry (Frozen) |
| Form | Purified |
| Classification | Monoclonal |
| Immunogen | Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with IgG from human serum. |
| Primary or Secondary | Secondary |
| Clone | 2C11cc |
PGC1 alpha Antibody, Alexa Fluor™ 532, Novus Biologicals™
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Rabbit Polyclonal Antibody
| Content And Storage | Store at 4°C in the dark. |
|---|---|
| Target Species | Goat,Hamster,Human,Mouse,Pig,Rat,Sheep,Squirrel |
| Host Species | Rabbit |
| Conjugate | Alexa Fluor 532 |
| Applications | ChIP Assay,Flow Cytometry,Flow Cytometry (Intracellular Staining),Gene Knock-Out,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Frozen),Immunohistochemistry (Paraffin),Immunoprecipitation,KnockDown,Western Blot |
| Form | Purified |
| Isotype | IgG |
| Research Discipline | Alzheimers Research, Cancer, Cardiovascular Biology, Cholesterol Metabolism, Chromatin Research, Diabetes Research, Epigenetics, Lipid and Metabolism, Mitochondrial Mediated Pathway, mTOR Pathway, Neuroscience, Transcription Factors and Regulators |
| Antigen | PGC1 alpha |
| Regulatory Status | RUO |
| Purification Method | Immunogen affinity purified |
| Dilution | Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow (Intracellular), Knockout Validated |
| Gene Alias | LEM6, Ligand effect modulator 6, ligand effect modulator-6, L-PGC-1alpha, peroxisome proliferative activated receptor, gamma, coactivator 1, alpha, peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B4-3ext, peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B4-8a, peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B5-NT, peroxisome proliferator-activated receptor gamma coactivator 1-alpha, peroxisome proliferator-activated receptor gamma, coactivator 1 alpha, PGC1, PGC-1(alpha), PGC1A, PGC-1-alpha, PGC1APGC-1(alpha), PGC1peroxisome proliferative activated receptor, gamma, coactivator 1, PGC-1v, PPAR gamma coactivator variant form, PPAR gamma coactivator-1, PPARgamma coactivator 1alpha, PPAR-gamma coactivator 1-alpha, PPARGC1, PPARGC-1-alpha |
| Gene ID (Entrez) | 10891 |
| Formulation | 50mM Sodium Borate |
| Classification | Polyclonal |
| Immunogen | This PGC1 alpha Antibody was developed against a recombinant protein made to an internal portion of the human PGC-1 alpha protein (within residues 400-550). [Swiss-Prot# Q9UBK2]. |
| Primary or Secondary | Primary |